A Sensitive and Selective Chromogenic Organic Reagent Diacetylmonoxime-3-amino-4-hydroxy Benzoyl Hydrazone Used for Direct and Derivative Spectrophotometric Determination of Copper(II)

Chem Sci Trans., 2015, 4(3), pp 838-843

DOI:10.7598/cst2015.1037

Research Article

A Sensitive and Selective Chromogenic Organic Reagent Diacetylmonoxime-3-amino-4-hydroxy Benzoyl Hydrazone Used for Direct and Derivative Spectrophotometric Determination of Copper(II)

Abstract

Diacetylmonoxime-3-amino-4-hydroxy benzoyl hydrazone (DMAHBH) was used as a novel chromogenic organic reagent for the determination of copper(II) by spectrophotometry. The novel chromogenic organic reagent diacetylmonoxime-3-amino-4-hydroxy benzoyl hydrazone (DMAHBH) gave bright yellow coloured water soluble complex with Cu(II) in basic buffer (pH 8.0-10.0) medium. The colour complex shows maximum absorbance at 412 nm. The system obeyed Beer's law in the concentration range of 0.3178 to 3.813 μ g/mL. The optimum copper(II) concentration range for accurate determination as evaluated from Ringbom?s plot was 0.6355 to 3.4953 μ g/mL. The molar absorptivity and Sandell's sensitivity were 1.65x10⁴ L.mol^-1cm^-1 and 0.00606 μ g/cm² respectively. The copper(II) forms M:L (I:I) colour complex with DMAHBH and stability constant of the complex was found to be. 7.15x10⁵. This developed method was applied for the determination of copper(II) in food samples like vegetables and milk samples and obtained with good results compared with reference AAS results.