Abstract

Evaluation of antioxidant activity of apple peel and pulp extracts by using different solvents was studied. Apple variety used for extraction was Gala. Apple peel and pulp extracts were prepared by using methanol, chloroform: acetone (4:1) and ethyl acetate solvents. The antioxidant activities of these extracts were determined by DPPH (1, 1- Diphenyl - 2- picryl hydroxyl) assay and RPA (reducing power assay) method. On comparing the activities of peel and pulp it was found that the activity of peel extracts was higher. It was found that free radical scavenging activity of both apple peel and pulp crude extracts was significantly higher in methanol extract among all the extracts studied. Reducing power assay (RPA) also gave significant results with methanol extract. All the extracts showed lower antioxidant activity compared to Gallic acid when used as standard.

Keywords

DPPH - (1, 1- Diphenyl - 2- picrylhydrazyl) assay, Reducing power assay, Antioxidant activity, Apple